DeterminationofHyperosideinAcanthopanaxsetchuenensisandA.leucorrhigusvar.fulvescensbyRP-HPLC
摘要: [Objective]HyperosideinAcanthopanaxsetchuenensisHarmsexDielsandA.leucorrhigusvar.fulvescensHarms&RehdwasidentifiedqualitativelybyLC-MS,andaRP-HPLCmethodwasestablishedtodeterminethecontentofhyperosideintheirdifferentmedicinalparts.[Methods]Theanalysiswascarriedoutat30℃onaKromasilC18columnelutedwithamobilephaseconsistedofmethanol(A)and0.5%phosphateinwater(B).Thedetectionwavelengthwas358nm,andtheflowratewas1.0mL/min.[Results]Thecalibrationcurvewaslinearintheconcentrationrangeof2.50-25.00μg/mL,theregressionequationwasY=4×10~6X+1.327×10~4(r=0.9999).Theaveragerecoverywas100.25%,withRSDof0.96%(n=9).[Conclusions]Thismethodwassimple,accurateandreplicable,anditwasusefulforthestudyofthequalitycontrolofA.setchuenensisHarmsexDielsandA.leucorrhigusvar.fulvescensHarms&Rehd. ...
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